yjbO [2017-11-27 17:43:42]

unknown

locus: BSU11620

pI: 6.55

mw: 31.32 kDa

protein length: 283 aa

gene length: 849 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]

Gene

Coordinates: 1,238,523 → 1,239,374

The protein

Catalyzed reaction/ biological activity

• RNA + uridine --> RNA pseudouridine (according to Swiss-Prot)

Protein family

• pseudouridine synthase rluA family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|064AE8CE6D52E35582F90654AA9548963573C05C|YhcT], [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|YlyB]

Structure

• [PDB|1V9F] (RluC from E. coli, 40% identical, aa 64 - 279)

Expression and Regulation

Operons

• genes: [gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]-[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-[gene|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]-[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
  description: [Pubmed|18067544]

  regulation

  • expressed during exponential growth

Biological materials

Mutant

• MGNA-B161 (yjbO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1160 NBRP B. subtilis, Japan]
• BKE11620 (Δ[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG, downstream forward: _UP4_GAGAATCATTGATTCTCTCC
• BKK11620 (Δ[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG, downstream forward: _UP4_GAGAATCATTGATTCTCTCC

References

• 18067544